Table 3.

Pros and cons of LAMP and traditional DNA barcoding methods

	PROS	CONS
LAMP	Target approach LAMP doesn’t require thermal cyclers Bst DNA polymerase has higher tolerance to sample inhibitors compared to traditional TAQ polymerase LAMP assay is fast (less than 30 min) High sensitivity and specificity LAMP can be carried out as colorimetric assay and the detection can be also done with naked eyes by measuring the turbidity or by using specific dyes	Primer design and optimization constraints Require specific reagents and equipments (e.g., detection system) Only one (or few) target species can be detected in the assay Unsuitable for unknown fungi
DNA barcoding of ITS region	Untargeted approach DNA Barcoding can be used to identify unknown fungi Several species can be detected	Time-consuming It generally requires DNA of higher quality compared to LAMP assay Require access to sequencing facilities/services