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. 2022 Mar 8;12:4132. doi: 10.1038/s41598-022-07954-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the pink-to-yellow/orange color change in the positive samples compared to the Negative Control, as a function of the RT-qPCR cycle threshold C_t. The quantification is performed by computing the color difference between the positive samples and the Negative Control, normalized to the difference between the Positive Control and Negative Control using the R, G, B linear dimensions defining the color space. The standard error bars represent the distribution of the normalized RGB distance for the samples categorized in four Ct bins of 0–25, 25–30, 30–35, and 35–40. (The figure is generated using MATLAB).