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. 2022 Mar 8;12:4132. doi: 10.1038/s41598-022-07954-2

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© The Author(s) 2022

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The top and side view images of the amplification in the tubes with and without the silicone oil layer. The silicone oil was not added to sample in the tube labeled (“A”), which shows condensation on the sidewalls and the cap. In contrast, the tube labelled as (“B”) contains 5 µl of silicone oil encapsulating the sample and preventing evaporation.