Table 4.
List of 25 OSCC tumor prediction biomarker candidate proteins (p < 0.01, FC > 2), and their detection in HNSCC cell line Secretomes and public saliva datasets
| Accession number | Gene symbol | Razor + unique peptides count | Protein name | p-value | OSCC tumor vs NAT FCs | HNSCC cell line secretomes average counts | Frequency of detection in 03 saliva datasets | SecretomeP prediction | Subcellular localization |
|---|---|---|---|---|---|---|---|---|---|
| P49588 | AARS | 29 | Alanine–tRNA ligase, cytoplasmic | 0.001 | 2 | 78 | 3 | NP | Cytosol |
| O15143 | ARPC1B | 18 | Actin-related protein 2/3 complex subunit 1B | 0.001 | 2 | 17 | 3 | NP | Cytoskeleton |
| P27708 | CAD | 38 | CAD protein;Glutamine-dependent carbamoyl-phosphate synthase;Aspartate carbamoyltransferase;Dihydroorotase | 0.000 | 4 | 45 | 3 | NP | Nucleus |
| P53618 | COPB1 | 35 | Coatomer subunit beta | 0.001 | 2 | 22 | 3 | NP | Cytosol |
| Q14019 | COTL1 | 15 | Coactosin-like protein | 0.006 | 2 | 38 | 3 | NCS | Endoplasmic reticulum |
| Q13217 | DNAJC3 | 18 | DnaJ homolog subfamily C member 3 | 0.000 | 3 | 18 | 3 | CS | Nucleus |
| Q9HC35 | EML4 | 21 | Echinoderm microtubule-associated protein-like 4 | 0.000 | 4 | 11 | 3 | NP | Cytoskeleton |
| Q9NZ08 | ERAP1 | 18 | Endoplasmic reticulum aminopeptidase 1 | 0.006 | 2 | 47 | 3 | CS | Cytosol |
| Q06210-2 | GFPT1 | 37 | Glutamine–fructose-6-phosphate aminotransferase [isomerizing] 1 | 0.000 | 4 | 6 | 3 | NP | Golgi apparatus |
| Q92896 | GLG1 | 31 | Golgi apparatus protein 1 | 0.001 | 2 | 34 | 3 | CS | Nucleus |
| Q9Y4L1 | HYOU1 | 37 | Hypoxia up-regulated protein 1 | 0.002 | 2 | 37 | 3 | CS | Plasma membrane |
| Q08380 | LGALS3BP | 11 | Galectin-3-binding protein | 0.001 | 3 | 269 | 3 | CS | Plasma membrane |
| P49257 | LMAN1 | 22 | Protein ERGIC-53 | 0.003 | 2 | 7 | 3 | CS | Plasma membrane |
| P61916 | NPC2 | 9 | Epididymal secretory protein E1 | 0.006 | 2 | 43 | 3 | CS | extracellular |
| P80303-2 | NUCB2 | 27 | Nucleobindin-2;Nesfatin-1 | 0.000 | 4 | 9 | 3 | CS | extracellular |
| Q02809 | PLOD1 | 20 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 | 0.001 | 3 | 74 | 3 | CS | Nucleus |
| O14974 | PPP1R12A | 20 | Protein phosphatase 1 regulatory subunit 12A | 0.004 | 3 | 14 | 3 | NP | Nucleus |
| Q13162 | PRDX4 | 11 | Peroxiredoxin-4 | 0.000 | 3 | 19 | 3 | CS | Nucleus |
| P78527 | PRKDC | 110 | DNA-dependent protein kinase catalytic subunit | 0.000 | 3 | 67 | 3 | NP | Nucleus |
| P46777 | RPL5 | 17 | 60S ribosomal protein L5 | 0.003 | 2 | 38 | 3 | NP | endoplasmic reticulum |
| Q9P2E9 | RRBP1 | 71 | Ribosome-binding protein 1 | 0.001 | 2 | 49 | 3 | NP | Plasma membrane |
| Q14683 | SMC1A | 36 | Structural maintenance of chromosomes protein 1A | 0.002 | 3 | 28 | 3 | NP | Nucleus |
| P42224 | STAT1 | 38 | Signal transducer and activator of transcription 1-alpha/beta | 0.000 | 3 | 28 | 3 | NP | Cytosol |
| P26639 | TARS | 29 | Threonine–tRNA ligase, cytoplasmic | 0.004 | 2 | 149 | 3 | NP | Plasma membrane |
| Q07157-2 | TJP1 | 27 | Tight junction protein ZO-1 | 0.004 | 2 | 32 | 3 | NP | Cytoskeleton |
CS Classically secreted, NCS Non-classically secreted, NP Not predicted to be secreted