Fig. 5.

MiR-214-5p downregulation obviously attenuated si-DANCR-induced protection against ox-LDL-induced endothelial injury. A, B VSMCs and HUVECs were transfected with si-DANCR, si-NC, or co-transfected with si-DANCR and inhibitor NC, or co-transfected with si-DANCR and miR-214-5p inhibitor, and then cells were treated with 50 μg/ml ox-LDL for 48 h. A CCK-8 assay. B Flow cytometry assay. C, D VSMCs and HUVECs were transfected with si-DANCR or si-NC, and then treated with 50 μg/ml ox-LDL for 48 h. COX20 expression was evaluated by qRT-PCR (C) and Western blot (D). The original images are shown in Additional file 1. Each experiment was repeated three independent times. **p < 0.01 vs. si-NC; ^##p < 0.01 vs. si-DANCR plus inhibitor NC group