Fig. 6.

DANCR/miR-214-5p/COX20 participated in development of AS in vivo. A Representative micrographs of the lesion areas in the aortic sinus were observed by H&E staining at ×400 magnification, scale bar = 50 μm. B, C Levels of inflammatory cytokines (B), MDA, and SOD (C) in blood samples of AS animal model were detected by specific detection kits. D Expression of DANCR, miR-214-5p, and COX20 in blood samples of AS animal model was evaluated by qRT-PCR. E, F Adenovirus overexpressing miR-214-5p and COX20 were injected into mice, and expression of miR-214-5p (E) and COX20 (F) in blood samples of AS animal model was evaluated by qRT-PCR. G Representative micrographs of lesion areas in the aortic sinus were observed by H&E staining. ×400 magnification, scale bar = 50 μm. There were six mice in each group. Each experiment was repeated three independent times. **p < 0.01 and ***p < 0.001