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. 2022 Feb 10;23(1):25. doi: 10.1186/s10194-022-01397-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Blocking S1PR1 signaling reduced the expression of CGRP and c-fos in the TCC. A, B Representative immunofluorescence staining images (A) and quantitative analysis of CGRP in different groups (B). Repeated daily injection of W146 reversed the increased immunoreactivity of CGRP induced by chronic NTG administration. n = 3/group; six sections from each mouse; *p < 0.05 vs. Saline group; ^###p < 0.001 vs. NTG group. Scale bars = 50 μm. C, D Double immunofluorescence staining images of c-fos (red) and DAPI (blue) in the TCC area, these results indicated daily administration of W146 decreased c-fos ir cells. n = 3/group; ***p < 0.001 compared with the Saline group; ^###p < 0.001 for the NTG + W146 group compared with the NTG group. Scale bars = 50 μm