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. 2022 Feb 19;27:16. doi: 10.1186/s11658-022-00316-w

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© The Author(s) 2022

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Fig. 1 — The establishment of the mouse model of ACD by oxazolone sensitization and challenge and evaluations. A Protocol for the establishment of the oxazolone (Oxa)-induced ACD model in mice. Control group receive vehicle (acetone) treatment only. B Typical pictures showing the neck skin condition of mice in vehicle- or oxazolone-treated group. C Pathological dermatitis scores of control and oxazolone group mice. D Bi-fold skin thickness evaluation of the neck skin of control and oxazolone group of mice. E Scratching behavior of mice treated with oxazolone or vehicle. F Representative pictures of hematoxylin and eosin (H&E) staining of neck skin of control and oxazolone group of mice. G Summary of epidermal thickness of control and oxazolone group mice. H Representative pictures of immunohistochemistry staining of CD3⁺ cells in neck skin of control and oxazolone group mice. I Summary of the number of CD3⁺ cells per observation field. n = 6 mice per group. **p < 0.01 versus control group. Scale bar, 100 μm