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. 2022 Feb 14;19:6. doi: 10.1186/s12014-022-09343-3

Table 1.

List of gene names and primer sequences used to conduct qPCR

Oligo name Forward sequence (5′–3′) Reverse sequence (5′–3′) Scale (µmole)
PDZD3 CCCTGTAAGTGCCCTGCTAT CTCCAGGGATCAGAAGGATCG 0.025 µmole
LTB4R TGGTACTTCCTCTCTGGCTGA CCATTGCAAGGACAGGCTTT 0.025 µmole
RASL10A TTGCATTTGGGGTAAACCTGGAA GCCACTCCTCCAAGCTTAATTC 0.025 µmole
SLC16A3 AGGTAACCTGAGACCTGGCT TGGTTCCGCGTCCCTGG 0.025 µmole
IL1B CCAATTCAGGGACCCTACCC GTTTTGGGTGCAGCACTTCAT 0.025 µmole
Beta actin TGTCATGGACTCTGGGGATG GGGCAGCTCGTAGCTCTTCT 0.025 µmole

A total of five genes were selected for qPCR validation of transcriptomics results, with beta actin serving as a control