Table 1.
Oligo name | Forward sequence (5′–3′) | Reverse sequence (5′–3′) | Scale (µmole) |
---|---|---|---|
PDZD3 | CCCTGTAAGTGCCCTGCTAT | CTCCAGGGATCAGAAGGATCG | 0.025 µmole |
LTB4R | TGGTACTTCCTCTCTGGCTGA | CCATTGCAAGGACAGGCTTT | 0.025 µmole |
RASL10A | TTGCATTTGGGGTAAACCTGGAA | GCCACTCCTCCAAGCTTAATTC | 0.025 µmole |
SLC16A3 | AGGTAACCTGAGACCTGGCT | TGGTTCCGCGTCCCTGG | 0.025 µmole |
IL1B | CCAATTCAGGGACCCTACCC | GTTTTGGGTGCAGCACTTCAT | 0.025 µmole |
Beta actin | TGTCATGGACTCTGGGGATG | GGGCAGCTCGTAGCTCTTCT | 0.025 µmole |
A total of five genes were selected for qPCR validation of transcriptomics results, with beta actin serving as a control