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. 2022 Jan 24;23(1):15. doi: 10.1186/s10194-021-01370-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Statistics of KCl-induced responses in the VPM under different conditions. A KCl application activated VGluT2 neurons in the ipsilateral VPM in awake mice (n = 50). When anaesthetized with isoflurane (0.6%, 0.9%, 1.2%, n = 10 respectively), or 50 mg/kg pentobarbital (n = 13), CSD did not significantly activate VGluT2 neurons in the VPM. Data are presented as means ± SD. B KCl application did not activate VGluT2 neurons in contralateral VPM (n = 15), or when the cortex was topical affected by NMDA receptor antagonist MK-801 (n = 8). When saline was applied ipsilaterally instead of KCl (n = 16), there was no significant activation. Data are presented as means ± SD. C The maximum average relative fluorescence change under different conditions. Data are presented as means ± SD. ***P<0.001 based on ANOVA between stimulated state and resting state in each case. D The maximum average relative fluorescence changes of 10 experimental mice under isoflurane anesthetized or awake state. Data are presented as means ± SD. *P<0.05 based on repeated measures ANOVA between awake state and 3 different levels of isoflurane anesthetized state. When anaesthetized with isoflurane, the activation of VGluT2 neurons in the VPM by CSD application was significantly decreased (P<0.05)