Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 24;27:8. doi: 10.1186/s11658-022-00306-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — β3GNT2 expression is controlled by miR-133b. A Prediction of regulatory miRNAs of β3GNT2. B Heatmap showing the top 50 miRNAs negatively correlated with β3GNT2 in ESCA. C Analysis of the correlation between miR-133b and β3GNT2 expression based on the TCGA database. D Correlation analysis of miR-133b and β3GNT2 expression in clinical ESCA samples. E Analysis of β3GNT2 mRNA expression by qPCR. F Analysis of β3GNT2 protein expression by Western blot. G Putative miR-133b binding site in the β3GNT2 3′-UTR. H Dual-luciferase reporter activity assay. I–L The proliferation, colony formation, migration, and invasion of cells co-transfected with β3GNT2 overexpression plasmid and miR-133b mimics were measured by CCK-8 assay (I), colony formation assay (J), Transwell migration assay (K), and Matrigel invasion assay (L). M Diagram of the proposed mechanism showing how β3GNT2 regulates ESCA progression. OV, cells transfected with pcDNA3.1/β3GNT2 plasmid. Data are expressed as mean ± SD. ^#P > 0.05, *P < 0.05; **P < 0.01