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. 2022 Mar 3;29(1):702–713. doi: 10.1080/10717544.2022.2044937

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Exo^R848+Ce6-mediated enhancement of antigen presentation of DC2.4 in vitro. (A) Schematic illustration of the experimental procedure. (B) Western blot analysis of Hsp70 in DC2.4 cells after treated with different formulations. GAPDH served as internal control. (C) Total ROS generation in DC2.4 cells after different treatments was detected by confocal microscope, scale bar = 100 μm. (D) Quantification of (C). (E, F) Flow cytometric analysis assessing the expression of CD80 and CD86 on DC2.4 cells in the various treatment groups. (G, H) Quantification of the enhanced maturation of DCs. Data are expressed as mean ± SEM of five independent biological samples. *p<.05, Exo^Ce6+US, Exo^R848, Exo^Ce6+R848+US versus Exo^Ctrl; ^#p<.05, Exo^Ce6+R848+US versus Exo^R848.