Figure 1.

HK-C. albicans primed BMDMs show increased antibacterial functions. a) Chronogram of the BMDM experiment. C57BL/6 WT mice were injected IP with an inducer of trained immunity in 100 μL before bone marrow cells were collected. Control mice were injected IP with the same volume PBS. Bone marrow cells were differentiated into bone marrow-derived macrophages (BMDMs) for six days, plated, and stimulated with Clostridium perfringens at a MOI of 5 or PBS vehicle. b) the killing ability of BMDM primed with PBS or HKCA (HK-C. albicans). 3 h post C. perfringens (MOI = 5) infection, viable bacteria were plated on BHI agar to determine CFU counts.

Data are shown as the mean ± SEM. n = 3 per group. Data from are three independent experiments. Statistical significance is indicated by *p < 0.05.