Figure 2.

Trained BMDMs increased release of extracellular traps. a) Extracellular DNA release from BMDM cultures was quantified. The release of extracellular DNA from BMDM (primed with PBS, 1 × 10⁶ CFU and 5 × 10⁶ CFU heat-killed C. albicans), was detected by SYTOX Green 2 h,3 h,4 h post-stimulation with the C. perfringens (MOI = 5). Extracellular DNA from cells was incubated without C. perfringens assumed as 1. Results are shown as fold increase relative to cells without C. perfringens. b) Western blot analysis of protein expression. Non-primed and HK-C. albicans-primed BMDMs were infected with C. perfringens (MOI = 5) or not for 3 h, whole cell lysates were analyzed for CitH3, PAD4 and GAPDH by Western blotting.

Data are shown as the mean ± SEM. n = 3 per group. Data from are three independent experiments. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.