Figure 3.

Mannan-Induced BMDM enhanced the memory response of ETs. a) Extracellular DNA release from BMDM cultures was quantified. BMDMs from non-primed and mannan-primed (1 mg/ml) mice were infected with C. perfringens (MOI = 5) or not. 3 h post-infection, the extracellular DNA in cell supernatants was stained with SYTOX Green and detected with a fluorescent reader. BMDMs incubated without C perfringens were used as control, assumed 1. Results are shown as fold increase relative to cells without C. perfringens. b) Western blot analysis of protein expression. Non-primed and mannan-primed BMDMs were infected with C. perfringens (MOI = 5) or not for 3 h, whole cell lysates were analyzed for CitH3, PAD4 and GAPDH by Western blotting.

Data are shown as the mean ± SEM. n = 3 per group. Data are pooled from 2 independent experiments. Statistical significance is indicated by *p < 0.05.