Figure 5.
Rapamycin inhibits the trained memory of ETs. Intraperitoneal injection in C57BL/6 WT mice with 4 mg/kg rapamycin for 30 min prior to HK-C. albicans (1 × 106 CFU) primed. Animals were sacrificed 3 days after injection and collected BMDMs. BMDMs infected with C. perfringens (MOI = 5) or PBS vehicle and fluorescence of extracellular DNA was measured 3 hours after infection. a) Extracellular DNA release was quantified. BMDMs stimulated with PBS were used as control. Fold changes in DNA release obtained in BMDM after stimulation with C. perfringens. the ratio of DNA release by C. perfringens versus PBS stimulation BMDMs is presented. b) Non-primed BMDMs infected without C. perfringens was used as control, was assumed to be 1. DNA release was quantified and presented as ratio vs control.
Data are shown as the mean ± SEM. n = 3 per group. Data are from three independent experiments. Statistical significance is indicated by *p < 0.05, **p < 0.01.