| Cell line (H. sapiens) |
Expi293F |
Thermo Fischer |
cat# A14527 |
SCN8A mutant transient transfections |
| Cell line (H. sapiens) |
FreeStyle 293 F |
Thermo Fischer |
cat# R710-07 |
Stable transfections |
| Strain, strain background (M. musculus) |
C57BL/6 J male Scn8aN1768D/+ (stock#400690)x C3HeB/FeJ female (strain#000658).Both Male and Female heterozygous Scn8aN1768D/+ micewere tested |
Licensed from Miriam Meisler, Univ. of Michigan.Wagnon et al., 2015
|
|
Colony maintained at The Jackson Laboratory |
| Strain, strain background (M. musculus) |
CF-1, male |
Charles River |
Code: 023 |
|
| Strain, strain background(R. norvegicus) |
Sprague Dawley, male |
Envigo |
Code: 002 |
|
| Chemical compound, drug |
NBI-921352 |
US Patent #10246453 B2 |
Compound ID #101 |
Synthesized at Xenon Pharmaceuticals |
| Chemical compound, drug |
Carbamazepine |
Sigma-Aldrich |
C4024 |
|
| Chemical compound, drug |
Phenytoin |
Sigma-Aldrich |
D4505 |
|
| Chemical compound, drug |
Lacosamide |
Toronto Research Chemicals |
L098500 |
|
| Gene (H. sapiens) |
SCN1A |
GenBank |
NM_006920 |
|
| Gene (H. sapiens) |
SCN2A |
GenBank |
NM_021007 |
|
| Gene (H. sapiens) |
SCN3A |
GenBank |
NM_0069220 |
|
| Gene (H. sapiens) |
SCN4A |
GenBank |
NM_000334 |
|
| Gene (H. sapiens) |
SCN5A |
GenBank |
NM_198056 |
|
| Gene (H. sapiens) |
SCN8A |
GenBank |
NM_014191 |
|
| Gene (H. sapiens) |
SCN9A |
GenBank |
NM_002977 |
|
| Gene (M. musculus) |
SCN1A |
GenBank |
NM_018733.2 |
|
| Gene (M. musculus) |
SCN2A |
GenBank |
NP_001092768.1 |
|
| Gene (M. musculus) |
SCN8A |
GenBank |
NM_001077499 |
|
| Gene (H. sapiens) |
SCN1B |
GenBank |
NM_199037 |
|
| Gene (H. sapiens) |
FGF13 |
GenBank |
NM_033642 |
|
| Gene (H. sapiens) |
CNTN1 |
GenBank |
NM_001843 |
|
| Recombinant DNA reagent |
pcDNA4/TO (vector) |
Thermo Fischer |
cat #V102020 |
Vector for SCNxA genes |
| Recombinant DNA reagent |
pcDNA6/TR(regulatory vector for tetracycline repressor protein) |
Thermo Fischer |
cat#V102520 |
Vector to generate inducible FreeStyle 293 F and Expi293F |
| Recombinant DNA reagent |
pcDNA3.1 (+)(vector) |
Thermo Fischer |
cat#V79020 |
Vector for SCN1B gene |
| Recombinant DNA reagent |
pcDNA3.1/Hygro(+) |
Thermo Fischer |
cat# V87020 |
Vector for FGF13 and CNTN1 genes |
