|
Cell line (Homo sapiens) |
ECPs (human chondro-progenitor cells) |
https://doi.org/10.3727/215517912X639324
https://doi.org/.10.3390/biom11020250
|
|
Fetal chondro-progenitor cell source (FE002-Cart.) established after standardized processing of a fetal cartilage sample in Prof Lee Ann Applegate lab in 2009. |
|
Antibody
|
Anti-TRPV4 (Rabbit polyclonal) |
Abcam
|
Cat#: ab191580
|
IF (1:200) |
|
Commercial assay or kit
|
Flou 4-AM (Calcium indicators) |
Thermo Fisher Scientific
|
Cat#: F14201
|
5 µM |
|
Chemical compound, drug
|
TRPV4 agonist; TRPV4 activator; GSK101
|
Sigma-Aldrich
|
Cat#: G0798
|
10 nM |
|
Chemical compound, drug
|
TRPV4 antagonist; TRPV4 inhibitor; GSK205
|
AOBIOUS
|
Cat#: AOB1612-5
|
10 µM |
|
Commercial assay or kit
|
NucleoSpin RNA XS
|
MACHEREY-NAGEL
|
Cat#: 740902.50
|
|
|
Commercial assay or kit
|
Live/dead (The Viability/Cytotoxicity Assay Kit) |
Biotium
|
Cat#: 30002
|
|
|
Commercial assay or kit
|
PrestoBlue; PB (Cell Viability Reagent) |
Thermo Fisher Scientific
|
Cat#: A13261
|
|
|
Software, algorithm
|
COMSOL Multiphysics
|
COMSOL Inc
|
COMSOL 5.4; RRID:SCR_014767
|
|
|
Software, algorithm
|
ImageJ
|
https://imagej.net/
|
RRID:SCR_003070
|
|
|
Software, algorithm
|
MATLAB
|
MathWorks
|
RRID:SCR_001622
|
|
|
Other
|
EGTA
|
Sigma-Aldrich
|
Cat#: 03777
|
|
|
Other
|
RGD peptide (motif for cell attachment via integrin binding) |
GL Biochem
|
|
GGGRGDS-NH2 This peptide was synthesized by GL Biochem
|
|
Other
|
4-Nitrophenyl chloroformate, NPC
|
Sigma-Aldrich
|
Cat#: 160210
|
|
|
Other
|
DAPI stain
|
Invitrogen
|
Cat#: D1306
|
2 µg/ml |
|
Other
|
TRIzol Reagent
|
Thermo Fisher Scientific
|
Cat#:15596026
|
|
|
Other
|
Hoechst 33258
|
Thermo Fisher Scientific
|
Cat#: H3569
|
0.2 µg/ml |
|
Other
|
Loading machine
|
Instron
|
Cat#: ElectroPuls E3000
|
|
|
Other
|
PID microprocessor
|
Minco
|
Cat#: CT16A
|
|
|
Other
|
Miniature RTD sensor
|
Minco
|
Cat#: S308
|
|
|
Other
|
Thermo-foil heater
|
Minco
|
Cat#: HM6975
|
|
|
Other
|
Gas mixer
|
ibidi
|
Cat#: 11922
|
|
