FIG 1.
Development of an animal model for studies of virulence properties of ACAM2000 and CL3. (A and B) CAST mice (n = 5) were infected with 106 PFU of ACAM-Luc or CL3-Luc i.n. (A) or i.p. (B). The progress of infection was measured by injecting luciferin and measuring luminescence. Ventral-view images of mice are shown from the indicated days postinfection using the same exposure time and binning factor. Red, blue, and purple denote intensity of luminescence from high to low. (C and D) Total photon flux (photons per second per square centimeter per steradian) of the entire mouse was calculated and shown for each group. Bars indicate arithmetic means ± standard deviations (SD). ****, P < 0.0001; ***, P = 0.0003. Significance was calculated using the Mann-Whitney test.