FIG 3.
ORF 212 is required for virulence of CL3. (A) The top diagram shows CL3-212tr-Luc GFP, with a truncation of ORF 212, followed by the separate GFP ORF regulated by the P11 promoter depicted, in blue. The lower two diagrams depict CL3-Δ213-Luc-mCherry and CL3-Δ214-216-Luc-GFP, with ORF 213 replaced with mCherry in red or ORFs 214 to 216 replaced with GFP in blue, each regulated by the P11 promoter. Yellow and green arrows indicate CL3 ORFs that were unaltered, as in Fig. 2A. (B) CAST mice (n = 5) were infected i.p. with 106 PFU of ACAM-Luc, CL3-Luc, or CL3 deletion viruses. The progress of infection was monitored by measuring luminescence on the indicated days, as described in the legend to Fig. 1. (C) Plots of average total photon flux of animals for each group. Bars indicate arithmetic means ± SD. ****, P < 0.0001; **, P < 0.01. Significance was calculated using Fishers exact test.