Table 5.
Univariate analysis of the association of immune-related factors with PFS and OS
| Factor | Group | n | PFS |
OS |
|||
|---|---|---|---|---|---|---|---|
| Median survival | p value | Median survival | p-value | ||||
| CD3 | ≧1253/mm3 | 24 | 8.4 (4.3–13.4) | 0.549 | 17.4 (7.8–23.1) | 0.837 | |
| <1253/mm3 | 24 | 8.1 (3.5–12.6) | 13.4 (7.6–23.1) | ||||
| CD4 | ≧539/mm3 | 24 | 8.4 (4.3–13.4) | 0.533 | 17.4 (9.7–23.8) | 0.158 | |
| <539/mm3 | 24 | 8.1 (3.6–11.6) | 13.1 (6.9–18.5) | ||||
| CD8 | ≧369/mm3 | 24 | 7.9 (4.2–11.6) | 0.436 | 15.1 (7.1–18.9) | 0.553 | |
| <369/mm3 | 24 | 9.0 (3.7–12.6) | 13.4 (8.0–23.9) | ||||
| Th1 | ≧109.3/mm3 | 24 | 8.8 (4.2–11.6) | 0.944 | 17.4 (13.4–23.1) | 0.235 | |
| <109.3/mm3 | 24 | 6.8 (3.5–12.6) | 9.7 (6.9–23.1) | ||||
| Th2 | ≧162.4/mm3 | 24 | 7.9 (4.2–11.6) | 0.708 | 15.1 (8.0–23.1) | 0.909 | |
| <162.4/mm3 | 24 | 8.8 (3.7–12.6) | 15.1 (6.6–23.9) | ||||
| Treg | ≧26.2/mm3 | 24 | 4.7 (3.3–9.3) | 0.00306* | 9.3 (7.1–17.4) | 0.0159* | |
| <26.2/mm3 | 24 | 11.6 (7.1-NA) | 18.9 (13.1-NA) | ||||
| ɤδT | ≧18.2/mm3 | 24 | 7.9 (4.2–12.6) | 0.529 | 17.4 (7.7–23.8) | 0.492 | |
| <18.2/mm3 | 24 | 8.8 (3.7–11.6) | 13.4 (7.8–18.5) | ||||
| NK | ≧129.3/mm3 | 24 | 8.4 (4.2–10.0) | 0.503 | 15.1 (7.9–18.9) | 0.278 | |
| <129.3/mm3 | 24 | 7.1 (3.7–13.4) | 17.4 (6.9-NA) | ||||
| NKT | ≧16.9/mm3 | 24 | 8.1 (4.3–9.3) | 0.493 | 15.1 (7.9–23.1) | 0.686 | |
| <16.9/mm3 | 24 | 8.8 (3.5–13.4) | 13.1 (6.9–23.9) | ||||
| MDSC | ≧6.2/mm3 | 24 | 7.9 (4.3–11.6) | 0.342 | 14.7 (7.8–18.9) | 0.363 | |
| <6.2/mm3 | 24 | 9.3 (3.7–13.4) | 18.5 (7.7–23.1) | ||||
| MUC1 ELspot | ≧12.8/105PBMC | 24 | 12.6 (9.3-NA) | <0.0001* | 23.1 (17.4-NA) | <0.0001* | |
| <12.8/105PBMC | 23 | 3.7 (3.2–4.9) | 7.6 (5.5–9.7) | ||||
| WT1 ELIspot | ≧9.3/104PBMC | 16 | 12.6 (6.8-NA) | 0.000914* | 23.9 (15.1-NA) | <0.0001* | |
| <9.3/104PBMC | 16 | 4.3 (3.3–8.8) | 7.6 (5.5–13.1) | ||||
| Skin reaction | ≧30 mm | 25 | 9.3 (7.1-NA) | 0.0107* | 18.9 (15.1–23.9) | 0.00174* | |
| <30 mm | 23 | 4.7 (3.6–9.2) | 7.9 (6.6–15.1) | ||||
Two groups were divided by the median value. Parenthesis represents a 95% confidence interval. The cutoff values for the skin reaction is determined on the basis of previous studies.17 CD3, CD3+T cell; CD4, CD4+T cell; CD8, CD8+T cell; Th1, type 1 helper T cell; Th2, type 2 helper T cell; Treg, regulatory T cell; ɤδT, T cell receptor ɤ and δ chain+ T cell; NK. Natural killer cell; NKT, natural killer T cell; MDSC, myeloid-derived suppressor cell; MUC1, Mucin1; WT1, Wilms’ tumor 1; ELIspot, enzyme-linked immunospot. *represents statistically significant.