TABLE 2.
Specific activities of the WT and its variants for 2-CN
| Mutants | Specific hydration activity (U/mg) (×10−3) | Specific hydrolysis activity (U/mg) (×10−3) | Total activity (U/mg) (×10−3) | Relative activity (%) |
|---|---|---|---|---|
| WT | 31.62 ± 0.93 | 5.48 ± 0.10 | 37.10 ± 1.03 | 100 |
| N165C | 12.07 ± 0.28 | 16.18 ± 0.65 | 28.25 ± 0.93 | 76 |
| N165A | Nda | 13.04 ± 0.23 | 13.04 ± 0.23 | 35 |
| N165G | Nd | 1.85 ± 0.05 | 1.85 ± 0.05 | 5 |
| W167A | Nd | 12.75 ± 0.22 | 12.75 ± 0.22 | 34 |
| W167G | Nd | 118.68 ± 4.15 | 118.68 ± 4.15 | 320 |
a
Nd indicates that the protein tested did not display hydration activity toward 2-CN.