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Specific activities (U/mg) of the WT and W167G toward nicotinonitrile and its chlorinated derivatives
| Mutants | Nicotinonitrile | 2-CN | 4-CN | 5-CN | 6-CN |
|---|---|---|---|---|---|
| WT | 20.36 ± 1.58 | 37.10 ± 1.03 (×10−3) | Nda | Nd | 0.90 ± 0.05 |
| W167G | 1.17 ± 0.03 | 118.68 ± 4.15 (×10−3) | Nd | Nd | 0.02 ± 0.01 |
Nd indicates that the protein tested did not display activity toward the substrate.
