TABLE 2.
Primers used in this study
| Primer | 5′→3′ | Product length (bp) | Function |
|---|---|---|---|
| FcodY-F | CCCAAGCTTGGGAAAAATAGGAGAACAAAGTGGCT | 809 | To construct codY overexpression strain FcodY |
| FcodY-R | CGCGGATCCGCGGTGGTGATGGTGATGATGCTTTTAGAAATTACGTCCTGCAA | ||
| codY-up-F | CCGCTCGAGTTTCTGAATTGATTGTTATGGC | 1,117 | To construct knockout codY strain |
| codY-up-R | AGCTTTGTTTAAACGGTCACTCCATCTTGCAAAA | ||
| codY-down-F | TCCCCCGGGAAAGCCAGGGTCTAAGCG | 1,128 | ΔcodY |
| codY-down-R | CGAGCTCAACCACCCATCGCAAACT | ||
| nisI-F | AAAGTATCCCGAGGTCTGAATG | 132 | qRT-PCR |
| nisI-R | CAACGGCAAATGCTTCAGTA | ||
| nisT-F | TTTCAAGAAGGGCGTCAACT | 243 | |
| nisT-R | CTGTCCATCTTTCATAACCACG | ||
| ilvD-F | GTCTATGGTGGAACGATTGAACA | 236 | |
| ilvD-R | GGATTGGAAGAGGAATAAGGTAAAC | ||
| codY-F | ATACGAAGGGCGAGTTGCT | 171 | |
| codY-R | CTGGGAAGATTGCTAATGGAC | ||
| EMSA-optS-F | CCAAGTGTTATTCAAAGGTTCA | 311 | EMSA |
| EMSA-optS-R | TGTTTTTGTGCCACAGGC | ||
| EMSA-ybbD-F | GCACAAGCAGCAGGTTTG | 318 | |
| EMSA-ybbD-R | ACCTAGTGCAAGAAAATCACG | ||
| EMSA-leuA-F | TTATGTCTACCTAAAGCAACAAAAT | 478 | |
| EMSA-leuA-R | TTCCAGTTGTTTAGCAATCGT | ||
| EMSA-ilvD-F | TAACTGAGAAAGGAAAGCAAAA | 305 | |
| EMSA-ilvD-R | ATTTCCGTCCCAATCCAT | ||
| EMSA-yncA/gltB-F | AAAATCAGGCTGCCAAAGT | 317 | |
| EMSA-yncA/gltB-R | AAAAGGGCATGGTTACGATA | ||
| EMSA-dtpT-F | TTTTCAAAGAATCTGTATAGGCA | 300 | |
| EMSA-dtpT-R | TTGAAAGATAGACAAGTGCACC | ||
| EMSA-codY-F | ATGCATGTGTGATTAATACGAAG | 510 | |
| EMSA-codY-R | CAGCAATGACAGAAGCGATA | ||
| EMSA-ydgC/D-F | CATTTGAATGGTTTTCCCAG | 304 | |
| EMSA-ydgC/D-R | ATTTCTAATAGATTTTGGTCGATT | ||
| EMSA-busR-F | TTTTTTGTCCAACTTTAAGTTCAT | 455 | |
| EMSA-busR-R | TGACTATCGTTCTCCAATTACAG | ||
| EMSA-pepN-F | ATAAATGACAAAATAGGGGATAAT | 181 | |
| EMSA-pepN-R | TTTACAGCCATGTTTTCTCCTA | ||
| EMSA-rmlA-F | TGGTTGCTCAACCAAGAAGA | 209 | |
| EMSA-rmlA-R | GACCTCCATAATCATAATTAATCAA | ||
| EMSA-ftsW1-F | ATTATCTTGCTGTTTTTTTATTTAT | 336 | |
| EMSA-ftsW1-R | TTCATTATCTTTTTTTCCTCAT | ||
| EMSA-ftsW-F | AAGAAGGCGTGAAATAGTGC | 397 | |
| EMSA-ftsW-R | TAGTTGCAAATCAATGAATAACTT | ||
| EMSA-murA-F | ATGGAAGGTACTTTTGTGTTATAAT | 526 | |
| EMSA-murA-R | AGCCTTAGCTTCAATATAACCAG | ||
| EMSA-murD-F | TCACTGACGACTTTATCTAATCTA | 310 | |
| EMSA-murD-R | TATTACGCTCTAAGTCTCATACTTT | ||
| EMSA-noxE-F | GAAACAATGTGGCAAGCA | 242 | |
| EMSA-noxE-R | ACCGATAACTACGATTTTCAT | ||