TABLE 3.
Oligonucleotides used in this study
| Oligonucleotide | Sequence 5′-3′a | Comments |
|---|---|---|
| oRH051 | TCAAAAAGCATATGTGCTATGC | LvbR_BS (fo) (39) |
| oRH068 | CTCATTTGGTGTGACTTCTAATCC | lvbR 1-201 (re), sequencing |
| oRH069 | CTATGGTCTTCAGCATAAAACAAATC | LvbR_BS (re) (39), sequencing |
| oRH104 | CCACATGAATTGTTCCATCAATGC | sequencing |
| oRH178 | CAAAACAATGCATCCCGCTGC | PlqsR-lqsR (fo) |
| oRH179 | GAGTGCCTGGTCAGACTTGC | PlqsR-lqsR (re) |
| oRH188 | GGAAACAGAATTCGAGCTCACTGCAATAAGCGCCTGCC | PlqsR (fo) (into SacI and XbaI site) |
| oRH189 | CATATGTATATCTCCTTCTTAAATCTAGAGGCTCCTCCTGAGCAAAACG | PlqsR (re) (into SacI and XbaI site) |
| oRH190 | GGAAACAGAATTCGAGCTCCCCCTTGATTGCAGAAGC | PlqsA (fo) (into SacI and XbaI site) |
| oRH191 | CATATGTATATCTCCTTCTTAAATCTAGACCATAACTCAGATTCTTTTCCC | PlqsA (re) (into SacI and XbaI site) |
| oRH199 | ATACAAGTACCTTTTGCTGGC | PlqsA-lqsA (fo) |
| oRH200 | CAATACCTGGAAGAGTTAGATGC | PlqsA-lqsA (re) |
| oRH201 | GAAACATGTATTCACCAAAGGC | PlvbR-lvbR (fo) |
| oRH202 | ATTAATTTTCCTTATCCTGCAAGC | PlvbR-lvbR (re) |
| oRH208 | ATGAAAAAACAAACCGACTTAATTG | lvbR ORF (fo) |
| oRH209 | TTATGAGGCTGAGTTTTTAGTTATTTTC | lvbR ORF (re) |
| oRH219 | AATAACCCTTTCCCTCCTGC | PlqsS-lqsS (fo) |
| oRH220 | CGCTATCTCAGTAAAAACGGC | PlqsS-lqsS (re) |
| oRH221 | ATTTTCCAGCAATAATTCGGC | Phnox1-hnox1 (fo) |
| oRH223 | GGAAACAGAATTCGAGCTCATCGCCAGAGGTTATCATGG | PlqsS (fo) (into SacI and XbaI site) |
| oRH224 | CATATGTATATCTCCTTCTTAAATCTAGATTCTTATCCTTAAATCCAGCTAAAAAACG | PlqsS (re) (into SacI and XbaI site) |
a
Regions overlapping with destination vector are underlined.