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Quantification of spores with different fates after 16 h of time-lapse tracking. B. subtilis PS832 spores in water were heated at 65, 70, 75, or 80°C for different times. Subsequently, spores were immobilized on a MOPS-agarose pad at 37°C with (10 mM each) AGFK (A) or 10 mM l-valine (B) for 16 h of time-lapse imaging. The numbers of individual spores examined by microscopy are given in Tables 1 and 2.
