FIG 1.

Effect of coincubation on virulence factor production Twitching (A), swimming (B), and swarming (C) motility, biofilm biomass (D), and pyoverdine production (E) of amoeba-adapted (black) and nonadapted (gray) P. aeruginosa isolates from days 3, 24, and 42. Rhamnolipid production of day 42 adapted and nonadapted isolates (F). Data are means and SEM. Statistical analyses for panels A, B, C, D, and E were performed using two-way ANOVA and Tukey’s multiple-comparison test. For panel F, statistical analyses were performed using an unpaired t test. *, P < 0.05; ***, P < 0.001.