FIG 2.

Effect of coincubation of P. aeruginosa with A. castellanii on virulence to C. elegans. C. elegans survival curves after exposure to P. aeruginosa isolates derived from amoeba-adapted (black) or nonadapted (gray) isolates taken from days 3 (A) and 42 (B) for 0, 4, 8, 24 and 48 h in a fast-kill assay. (C) Percent survival of C. elegans exposed to day 42 amoeba-adapted and nonadapted isolates of P. aeruginosa over 7 days in a slow-kill assay. Statistical significance between adapted and nonadapted isolates was determined using log-rank tests. The shaded area represents the 95% confidence interval (CI).