TABLE 1.
nsSNPs and indels found in known virulence factor-encoding genes that occurred solely in day 3 and 42 adapted populations
| Gene/locus tag | Function | Mutationa | Result on dayb: |
|
|---|---|---|---|---|
| 3 | 42 | |||
| Motility/adherence | ||||
| pilT/PA0395 | Twitching motility protein PilT | Δ1 bp (100) | − | + |
| Δ12 bp (570–581) | ||||
| pilI/PA0410 | Twitching motility protein PilI | V108G (GTG→GGG) | + | + |
| V109G (GTG→GGG) | ||||
| V123G (GTG→GGG) | ||||
| flgF/PA1081 | Flagellar basal body rod protein FlgF | E188* (GAG→TAG) | − | + |
| flgH/PA1083 | Flagellar basal body L-ring protein | G163C (GGC→TGC) | − | + |
| flgK/PA1086 | Flagellar hook-associated protein FlgK | Δ1 bp (797) | + | + |
| Y394* (TAC→TAG) | ||||
| fliS/PA1095 | B-type flagellar protein FliS | Δ2 bp (273–274) | + | − |
| fleS/PA1098 | Two-component sensor | T154P (ACC→CCC) | + | + |
| pilR/PA4547 | Two-component response regulator PilR | E253D (GAA→GAC) | − | + |
| pilN/PA5043 | Type 4 fimbrial biogenesis protein PilN | E148* (GAG→TAG) | − | + |
| pilM/PA5044 | Type 4 fimbrial biogenesis protein PilM | Δ2 bp (577–578) | − | + |
| Quorum sensing | ||||
| lasR/PA1430 | Transcriptional regulator LasR | Δ38 bp (480–517) | − | + |
| Iron uptake | ||||
| pvdN/PA2394 | Pyoverdine biosynthesis protein PvdN | L404P (CTG→CCG) | − | + |
| pvdL/PA2424 | Peptide synthase | A4026P (GCC→CCC) | + | − |
| fptA/PA4221 | Fe(III)-pyochelin outer membrane receptor | T239P (ACC→CCC) | + | − |
a
Δ, deletion mutation. nsSNPs are indicated with the position of the affected amino acid, and respective base changes are underlined. *, presence of translation termination (stop) codon.
b
+ and −, presence and absence of the respective mutations. The genes are classified according to the virulence factor database (http://www.mgc.ac.cn/VFs/main.htm).