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. 2022 Feb 25;49(4):54. doi: 10.3892/ijmm.2022.5110

Figure 5.

Figure 5

Myricetin causes cytoprotective autophagy in SK-BR-3 cells. (A) Western blot analysis of p-mTOR, beclin 1 and LC 3-II/I expression in SK-BR-3 cells after treatment with myricetin (10 and 20 µM). (B) Cell viability (as measured by an MTT assay) of SK-BR-3 cells pretreated with 1 mM 3-MA (autophagy inhibitor) for 1 h, followed by treatment with myricetin (10 µM for 24 h). (C) Western blot analysis of Bax, Bcl-2, and LC 3-II/I expression in SK-BR-3 cells. (D) Densitometric quantification of the bands in C. The control group (0 µM) was treated with the same amount of DMSO, and β-actin was used as a loading control. Data are displayed as the mean ± SD (n=3). *P<0.05 vs. control group; #P<0.05 vs. myricetin treatment group. MYR, myricetin; LC 3, microtubule-associated protein 1A/1B-light chain 3; MTT, 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide; 3-MA, 3-methyladenine; Bax, Bcl-2 associated X; Bcl-2, B cell lymphoma 2; DMSO, dimethyl sulfoxide.