Figure 4.

Wogonin regulates the protein expression of NOX². (A) Representative immunohistochemistry staining of NOX² in hippocampal CA1 area of the sham operation group (sham), TBI model group (TBI) and wogonin administration group (Wog) at 3 days. (B) Quantitative analysis of NOX² in the hippocampus. (C) NOX activity was detected in the hippocampus of TBI or sham-operated rats on days 1, 3 and 7. (D) Western blotting of NOX² protein level in hippocampal CA1 area was performed at 1, 3 and 7 days after the operation. The OD of the NOX² band at (E) 1, (F) 3 and (G) 7 days relative to β-actin were analyzed. Data are expressed as mean ± standard deviation (n=5/group). ^*P<0.05 vs. sham group; ^#P<0.05 vs. TBI group. TBI, traumatic brain injury; OD, optical density, RLU, relative light units.