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. 2022 Feb 17;49(4):52. doi: 10.3892/ijmm.2022.5108

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Copyright: © Hanusek et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Validation of the effects of TGF-β1 effects on the expression of predicted genes targeted by the miRNAs in Caki-2 cells. Caki-2 cells were treated (+TGF-β1) or not (-TGF-β1) with TGF-β1 for 48 h, before quantitative PCR analysis was performed to measure gene expression. The plots show the results of three independent biological experiments. Statistical analysis was performed using unpaired t-test. P<0.05 was considered statistically significant. ^*P<0.05, ^**P<0.01. miRNA/miR, microRNA; BMPR, bone morphogenetic protein receptor type; ETS, avian erythroblastosis virus E26 (V-Ets) oncogene homolog-1; PLD, phospholipase D.