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. 2022 Feb 17;49(4):52. doi: 10.3892/ijmm.2022.5108

Figure 9.

Figure 9

ETS1 regulates RCC proliferation in a RCC-tumor subtype dependent manner. (A) The effects of BMPR2 and ETS1 signaling in 786-O, KIJ265T, KIJ308T and Caki-2 cells. Plots show proliferation of cells analyzed using BrdU incorporation assay after silencing of BMPR2 (left) and ETS1(right). The graphs show mean results of 3 independent biological experiments performed in triplicate. (B) The expression of ETS1 in ccRCC and pRCC cells, the graphs were generated using TCGA transcriptomic data of KIRC (n=535) and KIRP (n=289) cohorts provided by ENCORI. (C) Left graph: Proliferation of 786-O, KIJ265T, KIJ308T and Caki-2 cell lines treated (+TGF-β1) or not (-TGF-β1) with TGF-β1 for 72 h. Right graph: ETS1 expression in 786-O, KIJ265T, KIJ308T and Caki-2 cell lines treated (+TGF-β1) or not (-TGF-β1) with TGF-β1 for 48 h. Statistical analysis was performed using paired t-test. *P<0.05; **P<0.01; ***P<0.001. ETS, avian erythroblastosis virus E26 (V-Ets) oncogene homolog-1; RCC, renal cell carcinoma; BMPR, bone morphogenetic protein receptor type; ccRCC, clear cell RCC; pRCC, papillary RCC; TCGA, The Cancer Genome Atlas.