. 2022 Feb 22;12:798350. doi: 10.3389/fmicb.2021.798350

TABLE 5.

Effects of dietary supplementation of Bacillus subtilis ATCC19659 on the mRNA abundance of GHR, TGF-β, IGF-1, IFN-γ, SOD, CAT, and GPX in liver and intestine tissue of broiler.

Treatments²	Items¹
	GHR	TGF-β	IGF-1	IFN-γ	SOD	CAT	GPX
Liver
CON	1.12^b	1.03^b	1.04^b	1.13^b	1.13^b	1.09^b	1.02^b
ZnB	0.5^c	1.02^b	0.99^b	1.49^b	1.14^b	1.65^b	1.02^b
BS-1	2.35^a	0.58^c	1.34^b	1.05^b	8.23^a	5.01^a	1.32^a
BS-3	2.22^a	1.46^a	2.12^a	2.4^a	7.47^a	4.81^a	1.07^b
BS-5	1.3^b	1.44^a	1.88^a	1.61^b	1.51^b	5.45^a	1.42^a
SEM	0.16	0.08	0.116	0.123	0.672	0.393	0.047
p-value	<0.001	0.001	<0.001	<0.001	<0.001	<0.001	0.002

Intestinal
CON	1.09^a	1.22^a	1.1^a	1.17^a	1.09	1.09	1.16^a
ZnB	0.63^b	0.76^b	1.17^a	0.92^b	0.9	0.84	0.64^b
BS-1	0.54^b	0.80^b	0.45^b	0.78^b	1.13	1.2	0.61^b
BS-3	0.57^b	0.72^b	0.59^b	0.85^b	0.96	0.77	0.62^b
BS-5	0.49^b	0.38^c	0.44^b	0.68^b	1.23	0.8	0.57^b
SEM	0.049	0.071	0.073	0.05	0.088	0.063	0.07
p-value	< 0.001	0.001	< 0.001	0.006	0.717	0.09	0.02

¹GHR, growth hormone receptor; TGF-β, transforming growth factor-beta; IGF-1, insulin like growth factor 1; IFN-γ, interferon γ; SOD, superoxide dismutase; CAT, catalase; GPX, glutathione peroxidase.

²CON, Chicks were fed a corn-and soybean-based diet; ZnB, chicks were fed basal diet containing 500 mg ZnB/kg; BS-1, chicks were fed basal diet containing 1 × 10⁸ CFU/g feed of BS-ATCC19659; BS-3, chicks were fed basal diet containing 3 × 10⁸ CFU/g feed of BS-ATCC19659; BS-5, chicks were fed basal diet containing 5 × 10⁸ feed CFU/g of BS-ATCC19659.

^a,b,cValues in the same row with different letter superscripts mean significant differences (p < 0.05). Data are presented as the mean ± SEM (n = 5).