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. 2022 Mar 3;25(3):280–284. doi: 10.1038/s41593-022-01023-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a) Top: drawing of zebrafish head delineating the position of the optic tectum and telencephalon as shown in panels (b) and (c). Bottom: Schematic of OPC laser ablation paradigm. b) Dorsal views of OPC reporter lines showing the optical tectum after unilateral OPC ablation using 2 photon laser pulses and quantification of OPC numbers per tectal hemisphere. Data are expressed as mean ± S.D. (44.8 ± 3.5 in control vs. 9.0 ± 4.0 in ablated animals at 1 dpa; 46.8 ± 3.7 in control vs. 15.1 ± 5.2 in ablated animals at 2 dpa; n=9 animals per group from three experiments). Scale bar: 50 µm. c) Dorsal views of OPC reporter lines showing the telencephalon after unilateral OPC ablation using 2 photon laser pulses and quantification of OPC numbers per tectal hemisphere. Data are presented as mean ± S.D. (25.4 ± 3.7 in control vs. 7.1 ± 1.4 in ablated animals at 1 dpa; 26.7 ± 3.6 in control vs. 7.7 ± 1.6 in ablated animals at 2 dpa; n=10 animals per group from four experiments). Scale bar: 50 µm. d) Schematic of OPC NTR ablation paradigm in panels e and f. e) Example images with OPC NTR ablation and quantification of OPC numbers at different time points after MTZ treatment of Tg(olig1:CFP-NTR) animals. Boxes represent the median (center line) and I.Q.R. (32±30/33 cells in control vs. 30±29/36 cells in NTR animals before MTZ treatment; 32±30/34 cells in control vs. 8±4/10 cells in NTR animals at 1 day after MTZ treatment; 34±32/36 cells in control vs. 1±0/3 cells in NTR animals at 3 days after MTZ treatment), and whiskers indicate the minimum and maximum values. n=8/14 animals in MTZ-control/OPC NTR ablation from three experiments. f) Left: 3D rotation view of RGC axon projections showing major projection layers in the tectal neuropil: stratum opticum (SO), stratum fibrosum et griseum superficiale (SFGS), stratum griseum centrale (SGC), stratum album centrale (SAC). Representative images from 12 animals in five independent experiments. Scale bar: 50 µm. Right: Overall layering of RGC projections remains intact after OPC ablation. Representative images from 7 animals in three experiments. Scale bar: 10 µm. g) Confocal images of individual RGC axon arbors at 7 and 10 dpf in control and OPC ablated animals (n numbers as in Fig. 3c). Arrowheads depict OPCs. Scale bars: 10µm. See Fig. 3c for traces.