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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Mol Biochem Parasitol. 2022 Jan 5;248:111447. doi: 10.1016/j.molbiopara.2022.111447

Figure 4. Pfcrisp− parasites grow normally as asexuals and undergo gametocytogenesis.

Figure 4.

(A) Ring stage synchronous cultures for WT PfNF54 and Pfcrisp− (clone 1 and 2) were set up at 1% parasitemia and parasite growth was measured over the course of two erythrocytic cycles using Giemsa-stained smears. Data were averaged from three biological replicates and presented as the mean ± standard deviation (SD). (B) Day 15 gametocytemia for WT PfNF54 and Pfcrisp− (clone 1 and 2) was measured using Giemsa-stained smears. Data were averaged from three biological replicates and presented as the mean ± standard deviation (SD). (C) Number of exflagellation centers per field at 15 min post-activation WT PfNF54 and Pfcrisp− (clone 1 and 2). Data were averaged from three biological replicates and presented as the mean ± standard deviation (SD). (D) Oocysts per mosquito midgut were enumerated on day 7 post feed for WT PfNF54 and Pfcrisp− (clone 1 and 2) fed mosquitoes. Data were averaged from three biological replicates with a minimum of 50 mosquito guts and presented as the mean ± standard deviation (SD). NS-Not significant.