Figure 1.

Representative gating strategy for the T lymphocyte maturation stages and T_REG panels. Gating strategy used to analyze the percentages of the different CD4⁺ and CD8⁺ T cell subsets based on the expression of the markers CD3, CD4, CD8, CD45RA and CCR7, and of CD127, CD25, CD45RO, CCR4 and HLA-DR for T_REG subsets within CD4⁺ T cells. CD31 and PTK7 were used to characterize RTEs. Positivity controls for CCR7 vs CD45RA using lymphocytes, for CD45RO vs CCR4 using CD4^- T cells and for CD45RO vs HLA-DR using CD4⁺ T cells as internal reference populations. FMO control for PTK7 was used.