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. 2022 Feb 23;16:839118. doi: 10.3389/fncel.2022.839118

Figure 2.

Figure 2

Cre-inducible LCN2 overexpression in hippocampal astrocytes of LCN2-cTg mice. (A) An illustration of adeno-associated viral (AAV) vector containing the Cre construct (AAV-GFAP-mCherry and AAV-GFAP-mCherry-Cre). The expression of mCherry or mCherry-Cre is controlled by GfaABC1D, a shortened version of the GFAP promoter (upper). A schematic, showing the route of AAV-GFAP-mCherry-Cre administration into the hippocampal CA1 region and experimental timeline (lower). (B) Following the administration of AAV-GFAP-mCherry-Cre, brain tissue sections were subjected to immunofluorescence analysis to localize the expression of mCherry-Cre (red), LCN2 (blue), and GFP (green) in astrocytes (GFAP, magenta), microglia (Iba-1, magenta), and neurons (NeuN, magenta). Arrowheads (yellow) indicate the colocalization of GFAP, mCherry-Cre, LCN2, and GFP. (C) We further compared the LCN2 expression (magenta) in the hippocampal region of LPS-injected and LCN2-cTg mice (n = 6). DAPI for nuclei (blue). The quantification of colocalization is shown in the adjacent graphs (means ± SEM (*p < 0.05, between the indicated groups). n.d., not detected; n.s., not significant; a.u., arbitrary unit.