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. 2022 Mar 8;13(3):217. doi: 10.1038/s41419-021-04446-5

Fig. 7. miR-556-3p/CLIC1/PI3K/AKT axis manipulates the viability, invasion, and migration of GC cells.

Fig. 7

A The protein expression of CLIC1, PI3K, and AKT and phosphorylation of PI3K and AKT after overexpression or inhibition of miR-556-3p detected by Western blot analysis. B The protein expression of CLIC1, PI3K, and AKT and phosphorylation of PI3K and AKT after overexpression miR-556-3p and CLIC1 determined by Western blot analysis. C Western blot analysis was used to detect the effect of AKT inhibitor LY294002 on the protein expression of PI3K and AKT and phosphorylation of PI3K and AKT. D CCK-8 was used to detect cell proliferation. E Cell migration detected by Transwell assay. F The invasion of GC cells measured by Transwell assay. * represents the comparison between two groups. *p < 0.05; **p < 0.01; ***p < 0.001. All experiments were repeated 3 times.