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. 2022 Mar 8;12:3794. doi: 10.1038/s41598-022-07867-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Uninfected Vero E6 cells (A1, A2, A3 and B1, B2, B3 are progressive zoom-in of two different group of cells). Uninfected cells do not fuse during growth and when progressing toward confluency. Coarse filopodia (blue arrows) and caveolae (red arrows) are present in these cells. There are bridge points of contact (green arrows). Vesicles can be seen over the cell surface (yellow arrows) or close to the cells. In (A3), a 200 nm vesicle is seen budding or merging at the cell membrane level. (B3) Likely captured the exosomes discharge from a multivesicular body.