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. 2022 Mar 8;12:3794. doi: 10.1038/s41598-022-07867-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TNT from a polarized infected cell. Some infected cells start to bulge and detach from the floor, while emitting TNT in a specific bipolar fashion. An asymmetry in these bipolar cells is often noted, so that there is a growth cone (b, 2) on one side, that may emit a slight prolongation of variable length (a, 1), while the opposite TNT (4) ends with a polymorphic bulge (d, 5). Breakages at TNT origin close to the cell body visualize an inner content of cylindrical structures of about 25 nm in diameter, suggestive of microtubules (c). A corrugated surface (e) and apoptotic bodies (f) are specific to this infected cell stage (All photomicrographs at 10,800×; apart from c top, 135,000× and c bottom 270,000×).