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. 2022 Mar 8;5:211. doi: 10.1038/s42003-022-03149-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — (a–d) In vitro cleavage activities of CjCas9 (a, b) and enCjCas9 (c, d) toward DNA targets with different PAMs. The linearized plasmid targets were incubated with the CjCas9–sgRNA complex at 37 °C for 2 min. Data are mean ± s.d. (n = 3). (e, f) Sequence logos of CjCas9 (e) and enCjCas9 (f) obtained from the in vitro PAM discovery assay. (g, h) 2D PAM profiles of CjCas9 (g) and enCjCas9 (h) obtained from the in vitro PAM discovery assay.