Table 5.
Ingredients, dietary formulations (g kg−1) and analysed proximate compositions of the control and experimental diets.
| Ingredients | LM0 | LM5 | LM10 | LM15 | LM20 | ||
|---|---|---|---|---|---|---|---|
| Soybean meal | 500.0 | 475.0 | 450.0 | 425.0 | 400.0 | ||
| Lemna minor | – | 50.0 | 100.0 | 150.0 | 200.0 | ||
| Wheat flour | 245.0 | 223.0 | 201.0 | 179.0 | 158.0 | ||
| Corn flour | 147.0 | 147.0 | 146.0 | 146.0 | 146.0 | ||
| Sunflower oil | 62.0 | 58.0 | 55.0 | 51.0 | 47.0 | ||
| Vitamin/minerals premix1 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | ||
| Mono calcium phosphate | 20.0 | 21.0 | 23.0 | 24.0 | 25.0 | ||
| Choline chloride | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | ||
| Histidine | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | ||
| Methionine | 12.0 | 12.0 | 11.0 | 11.0 | 10.0 | ||
| Lysine | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | ||
| Threonine | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Polynomial contrasts | |||||||
|---|---|---|---|---|---|---|---|
| Linear | Quadratic | ||||||
| Proximate analysis (% dry matter basis) | |||||||
| Moisture | 6.34 ± 0.08a | 6.38 ± 0.10a | 6.20 ± 0.02a | 6.27 ± 0.04a | 6.21 ± 0.5a | 0.089 | 0.902 |
| Crude protein | 31.61 ± 0.14a | 32.05 ± 0.16a | 31.42 ± 0.15a | 31.68 ± 0.21a | 31.63 ± 0.09a | 0.285 | 0.796 |
| Crude lipid | 7.42 ± 0.06a | 7.44 ± 0.12a | 7.48 ± 0.13a | 7.35 ± 0.04a | 7.32 ± 0.05a | 0.324 | 0.444 |
| Carbohydrate | 47.51 ± 0.08a | 46.59 ± 0.10a | 47.19 ± 0.09a | 47.00 ± 0.09ab | 46.02 ± 0.07b | 0.003 | 0.257 |
| Crude ash | 7.22 ± 0.22a | 7.54 ± 0.07a | 7.72 ± 0.04a | 7.70 ± 0.04a | 7.82 ± 0.08a | 0.001 | 0.077 |
| Energy value (Kcal 100 g−1) | 382.52 ± 0.36a | 381.52 ± 0.44a | 381.73 ± 0.58a | 381.79 ± 0.60a | 377.38 ± 0.31a | 0.472 | 0.075 |
LM0 = Control, soybean; LM5 = 5% L. minor; LM10 = 10% L. minor; LM15 = 15% L. minor; LM20 = 20% L. minor.
1Supradyn multivitamin tablets (Piramal Enterprises Ltd., Mumbai, India) containing minerals and trace elements (as mg/kg in diets): = Vitamin A (as acetate) 12; Cholecalciferol 0.1; Thiamine mononitrate, 40; Riboflavine 40; Pyridoxine hydrochloride, 12; Cyanocobalamin, 0.06; Nicotinamide, 400; Calcium pantothenate 65.20; Ascorbic acid 600; α-Tocopheryl acetate,100; Biotin, 1.00. Minerals: Tribasic calcium phosphate, 516; Magnesium oxide, 240; Dried ferrous sulphate, 128.16; Manganse sulphate monohydrate 8.12; Total phosphorus, 103.20. Trace elements: Copper sulphate pentahydrate 13.56; Zinc sulphate, 8.80; Sodium molybdate dihydrate, 1.00; Sodium borate 3.52.