Figure 4.

The assessment of mitochondrial translation in HEMK1 KO cells. (A) Representative gel images of total and nascent mitochondrial proteins. Infrared (IR)-800 dye was conjugated to HPG-containing newly synthesized proteins. Total protein was stained with CBB. A black line is inserted between lanes 4 and 5 (counting from the left-hand side) because they were not adjacent lanes, although these lanes are originated from the same gel. The original image is shown in Figure S6A. (B) Bar graph of HPG signal/CBB signal for (A). Error bars represented the mean ± SEM calculated from samples shown in Figure S6A (see detailed measurements in Figure S6A). The Student’s t test, two-tail, was used for testing the statistical significance.