Table 2.
Read parameters of the two scRNA-seq approaches CellenONE-ICELL8 and ICELL8 (CogentDS).
| QC-Parameters | CellenONE-ICELL8 | ICELL8 |
|---|---|---|
| Samples | 8 | 8 |
| No. of cells | 5184 | 3616 cells |
| No. of cells Post-QC | 3135 | 3129 cells |
| Total Reads | 1.67G | 1.01 G |
| Barcoded Reads | 1.61G | 985 M |
| Fraction Barcoded Reads | 0.96 | 0.98 |
| Barcodes Identified | 5184 cells | 3616 cells |
| Reads per Barcode | 309.84 K | 272.54 K |
| Barcoded Reads | 1606229682 (100%) | 985522031 (100%) |
| Trimmed Reads | 1601535800 (99.71%) | 982927397 (99.74%) |
| Unmapped Reads | 86672148 (5.4%) | 16885303 (1.71%) |
| Mapped Reads | 1514863652 (94.31%) | 966042094 (98.02%) |
| Uniquely Mapped Reads | 1270205342 (79,08%) | 778278333 (78.97%) |
| Multimapped Reads | 244658310 (15,23%) | 187763761 (19.05%) |
| Total Exon Reads | 788938677 (49,12%) | 727757440 (73.84%) |
| Unique Exon Reads | 714643829 (44.49%) | 654119292 (66.37%) |
| Ambiguous Exon Reads | 74294848 (4.63%) | 73638148 (7.47%) |
| Total Intron Reads | 337929018 (21.04%) | 37383326 (3,79%) |
| Unique Intron Reads | 300416455 (18,7%) | 32630018 (3.31%) |
| Ambiguous Intron Reads | 37512563 (2.34%) | 4753308 (0.48%) |
| Intergenic Reads | 143337647 (8.92%) | 13137567 (1.33%) |
| Mitochondrial Reads | 93167354 (5.8%) | 57107478 (5.79%) |
| Ribosomal Reads | 30369153 (1.89%) | 29861198 (3.03%) |