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. 2022 Mar 8;13:1213. doi: 10.1038/s41467-022-28832-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Upper panels: V_nucfree (A), V_prehyd (B), V_3nuc (C), and V_2nuc (D) viewed from the cytosolic side. The scale bar is 20 Å. Magnified views of the three nucleotide-binding sites (AB_open, AB_semi, and AB_closed) in each structure are shown in the rows below. Cryo-EM maps are represented as semi-translucent. Bound nucleotides and Mg ions are shown in ball-and-stick and sphere representation, respectively. The scale bar is 4 Å.