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. 2022 Mar 5;3(1):101220. doi: 10.1016/j.xpro.2022.101220

Table 2.

The PCR cycling conditions for each 16S rRNA gene qRT-PCR assay

PCR cycling conditions
Steps Temperature Time Cycles
Initial denaturation 95°C 10 min 1
Denaturation 95°C 15 s 35–40 cycles
Annealing & extension 60°C 60 s
Final extension 72°C 10 min
Hold 4°C forever 1