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. 2022 Feb 23;16:843731. doi: 10.3389/fncir.2022.843731

FIGURE 2.

FIGURE 2

Crawling in reduced experimental configurations. (Ai) Left, diagram depicting the recording configuration; right, representative extracellular recording of a DP nerve in an isolated ganglion (G0) during a dopamine-induced crawling episode. (Aii) As in (Ai) for a chain of three ganglia (G1-G2-G3) where a DP nerve was recorded in each one. (B) Box plots describing the cycle frequency, duty cycle and firing frequency in G0, G1, G2, and G3; n = 12 ganglia from 11 leeches for G0, 15 ganglia from 11 leeches for G1, 16 ganglia from 14 leeches for G2, 13 ganglia from 10 leeches for G3. Comparison of G0 vs. G2 was performed by Wilcoxon rank-sum test; # indicates p = 0.027. For comparison of G1, G2, and G3 we used Kruskal-Wallis test; p > 0.05 for the three variables. (C) Dot plot describing the coefficient of variation (CV) of the cycle frequency, duty cycle and firing frequency in G0 and G2 [n as in panel (B)]. Each dot presents the CV value of the 10 cycles analyzed in each preparation. # indicates p < 0.001 (Wilcoxon rank-sum test).