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. 2022 Mar 8;13:1209. doi: 10.1038/s41467-022-28549-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Volcano plot depicting differentially expressed genes in CMTr1^13A; CMTr2^M32 double mutant flies compared to control flies. b Functional classification of upregulated (bottom) and downregulated (top) genes in CMTr1^13A; CMTr2^M32 double mutant flies compared to control flies. c Incubation of monophosphorylated RNA (pRNA, gray) and capped RNA with (white) or without 2′-O-ribose methylation of cap-adjacent nucleotides (black) in nuclear (nu) and cytoplasmic (cy) extracts from S2 cells. M: length in nucleotides. The graph to the right depicts the percent undegraded RNA left after 45 min as mean ± SE (n = 5, except n = 4 for pRNA, p = 0.03 by one way ANOVA followed by Tukey’s test). Source data for gel and RNA stability values are provided as a Source Data file.