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. 2022 Mar 8;12:3732. doi: 10.1038/s41598-022-07796-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Linear discriminant analysis effect size (LEfSe) analysis identified the most differential microbiota between G0 and G1 (A), and G0 and G2 (B). The LDA score also indicates the effective size and ranking of each differentially abundant taxon (LDA score > 3.0; alpha value p < 0.05). Plots were depicted using LEfSe from the Galaxy platform of the Huttenhower lab. G0: no renal stones (control); G1: incidental renal stones; and G2: prevalent renal stones.